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BP000728-ENZ-286: Recombinant T4 DNA Ligase
Source: Escherichia Colilambda lysogen NM 989-derived.
Incubation of a 50µl reaction containing 13,000 units of T4 DNA Ligase with 1µg of a mixture of single and double-stranded [3H] E. coli DNA (200,000 cpm/ug) for 4 hours at 37C released < 0.3% of the total radioactivity.
One Weiss unit is equivalent to circa 67 cohesive-end ligation units.T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200 mM.
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